Spatial transcriptomic profile of scleroderma skin. (a) UMAP (Uniform Manifold Approximation and Projection) visualization of SpaceRanger clusters 0–5. (b) Loupe Browser visualization of clustering via SpaceRanger pipeline presenting distinct regions within the histological section of scleroderma skin. (c) Cell type composition of each cluster based on prediction score from single-cell transcriptomic clustering. (d) Heatmap of differential mitochondrial gene expression across six Seurat clusters in systemic sclerosis. Rows represent individual mitochondrial genes, distinguishing nuclear DNA-encoded and mitochondrial DNA-encoded subunits, with clusters (0–5) on the x-axis showing average log2 fold changes. NK, natural killer.