Loss of Lats1 and Lats2 activates an inflammatory/immune response in adrenal glands of Lats1^flox/flox;Lats2^flox/flox;AS^cre/+ mice. (A) GSEA analysis of RNA sequencing gene expression of inflammation and macrophage-related gene sets in the adrenal cortex of 4-week-old Lats1^flox/flox;Lats2^flox/flox;AS^cre/+ male mice compared to the adrenal cortex of Lats1^flox/flox;Lats2^flox/flox mice. (B) RT-qPCR analyses of genes involved in the inflammation/immune response in the adrenal gland of 4-week-old males of the indicated genotypes. RT-qPCR data were normalized to the housekeeping gene Rpl19 and are expressed as means (columns) ± SEM (error bars). Asterisks = significantly different from control (*P < .05; **P < .01; ***P < .001; ****P < .0001). (C) Immunohistochemical analysis of SCARA1 expression in adrenal glands from 4-week-old males of the indicated genotypes. Arrow = SCARA1+ cells in the ECM.

Abbreviations: ECM, extracellular matrix; GSEA, Gene Set Enrichment Analysis.