Simulation of GFP production for all promoter architectures using base parameter values. (a) The concentration of GFP over time for each system. The promoter architectures are grouped by the different gRNA target site strategies (Heterogeneous vs Identical). Systems with no gRNA control and with a single gRNA target site are also included. (b) The fold repression for each promoter architecture, comparing the GFP concentrations shown in (a) for each repressed system against the Base Expression (No Regulation) system. Bar height indicates the mean fold repression between 72 and 96 h in the time course.