Complementation of miPEP858^CR phenotype by functional truncated peptide via miR858-associated pathway. A) Representative image of 10-d-old seedlings of WT and miPEP858^CR lines (L-1, L-3, L-5) grown on half-strength MS medium supplemented with water (control) and 0.25 µM 1H, 2H, and miPEP858a. Scale bar, 1 cm. B) Root lengths of 10-d-old seedlings of WT and miPEP858^CR lines (L-1, L-3, L-5) grown on half-strength MS medium supplemented with water (control) and 0.25 µM 1H, 2H, and miPEP858a (n= 30 independent seedlings, indicated by the small open circles). The experiment was repeated 3 times independently, with similar results. C) Representative image of 10-d-old seedlings of WT and miPEP858^CR lines (L-1, L-3, L-5) grown on half-strength MS medium supplemented with water (control) and 0.25 µM 1F, 2F, and 3F. Scale bar, 1 cm. D) Root lengths of 10-d-old seedlings of WT and miPEP858^CR lines (L-1, L-3, L-5) grown on half-strength MS medium supplemented with water (control) and 0.25 µM 1F, 2F, and 3F (n= 30 independent seedlings, indicated by the small open circles). The experiment was repeated 3 times independently, with similar results. E) Representative image of 30-d-old WT and miPEP858^CR lines (L-1 and L-3) supplemented with water (control) and 0.25 µM 2H, 3F, and miPEP858a. F) Bolting time of WT and miPEP858^CR lines (L-1 and L-3) grown under standard light conditions (16-h light/8-h dark) supplemented with water (control) and 0.25 µM 2H, 3F, and miPEP858a. n = 15 (the small open circles represent the individual values). G) Rosette diameter of 30-d-old WT and miPEP858^CR lines (L-1 and L-3) supplemented with water (control) and 0.25 µM of 2H, 3F, and miPEP858a. The statistical analysis was performed using 2-tailed Student's t-tests. The data are plotted as means ± SD. The error bars represent SDs. The asterisks indicate significant differences; *P < 0.1; **P < 0.01; ***P < 0.001.