Modulation of expression of miR858 and target genes and metabolite accumulation on supplementation with truncated peptide. A) Quantification of miR858a and its target genes MYB12 and CHS in 5-d-old seedlings of WT grown on half-strength MS medium supplemented with water and miPEP858^CR lines supplemented with water (control) and 0.25 µM of truncated peptides 2H and 3F. B) Western blot analysis of MYB12 and CHS protein in 5-d-old WT seedlings grown on half-strength MS medium supplemented with water and miPEP858^CR lines supplemented with water (control) and 0.25 µM of 2H, 3F, and miPEP858a. Actin was used as the loading control. C) Quantification of lignin in 35-d-old stems of WT plants supplemented with water and miPEP858^CR lines supplemented with water (control) and 0.25 µM of 2H, 3F, and miPEP858a. The experiments were repeated 3 times independently, with similar results. D) Lengths of vascular bundles of 35-d-old stems of WT plants supplemented with water and miPEP858^CR lines supplemented with water (control) and 0.25 µM of 2H, 3F, and miPEP858a (n = 15). E) Lengths of interfascicular fibers of 35-d-old stems of WT plants supplemented with water and miPEP858^CR lines supplemented with water (control) and 0.25 µM of 2H, 3F, and miPEP858a (n = 15). F) Quantification of total flavonol in 10-d-old WT seedling grown on half-strength MS medium supplemented with water and miPEP858^CR lines supplemented with water (control) and 0.25 µM of 0.25 µM of 2H, 3F, and miPEP858a. FW, fresh weight. G) Quantification of anthocyanin in 10-d-old WT seedlings grown on half-strength MS medium supplemented with water and miPEP858^CR lines supplemented with water (control) and 0.25 µM of 0.25 µM of 2H, 3F, and miPEP858a. FW, fresh weight.