Figure 7.
Effect of truncated peptide on downstream processes to regulate growth and development. A) Histochemical staining showing GUS activity in 5-d-old transgenic seedlings of Pro:ATG1::GUS grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. Scale bars, 1,000 µm. The experiment was repeated 3 times independently (n = 15 seedlings), with similar results. B) Relative expression of GUS in Pro:ATG1:: GUS transgenic seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. C) Histochemical staining showing GUS activity in 5-d-old transgenic seedlings of Pro:ORF1::GUS grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. Scale bars, 1,000 µm. The experiment was repeated 3 times independently (n = 15 seedlings), with similar results. D) Relative expression of GUS in Pro:ORF1::GUS transgenic seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. E) Quantification of AtPSK4 in 5-d-old WT seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a exogenously. F) Relative expression of auxin genes (AUX1, PIN1, PIN2, ABCB19, and YUC1) in 5-d-old WT seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a. G) Relative expression of EXPA2, EXPA11, and EXPA15 gene expression in 5-d-old WT seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a. H) Histochemical staining showing GUS activity in 5-d-old transgenic seedlings of Pro:PSK4::GUS lines (L-1 and L-2) grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a. Scale bars, 1,000 µm. The experiment was repeated 3 times independently (n = 15 seedlings), with similar results. I) Relative expression of GUS in seedlings of Pro: PSK4:: GUS transgenic lines (L-1 and L-2) grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a.

Effect of truncated peptide on downstream processes to regulate growth and development. A) Histochemical staining showing GUS activity in 5-d-old transgenic seedlings of Pro:ATG1::GUS grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. Scale bars, 1,000 µm. The experiment was repeated 3 times independently (n = 15 seedlings), with similar results. B) Relative expression of GUS in Pro:ATG1:: GUS transgenic seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. C) Histochemical staining showing GUS activity in 5-d-old transgenic seedlings of Pro:ORF1::GUS grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. Scale bars, 1,000 µm. The experiment was repeated 3 times independently (n = 15 seedlings), with similar results. D) Relative expression of GUS in Pro:ORF1::GUS transgenic seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a. E) Quantification of AtPSK4 in 5-d-old WT seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of 1H, 2H, 1F, 2F, 3F, and miPEP858a exogenously. F) Relative expression of auxin genes (AUX1, PIN1, PIN2, ABCB19, and YUC1) in 5-d-old WT seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a. G) Relative expression of EXPA2, EXPA11, and EXPA15 gene expression in 5-d-old WT seedlings grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a. H) Histochemical staining showing GUS activity in 5-d-old transgenic seedlings of Pro:PSK4::GUS lines (L-1 and L-2) grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a. Scale bars, 1,000 µm. The experiment was repeated 3 times independently (n = 15 seedlings), with similar results. I) Relative expression of GUS in seedlings of Pro: PSK4:: GUS transgenic lines (L-1 and L-2) grown on half-strength MS medium supplemented with water (control) and 0.25 µM of functional truncated peptides 2H and 3F and miPEP858a.

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