Downregulation of mitochondrial transcription factor A (TFAM) impeded epithelial-mesenchymal transition (EMT) of HTR-8/SVneo cells and BeWo cells. A, The protein levels of E-cadherin and N-cadherin were detected by Western blot in villi from the control group (n = 6) and polycystic ovary syndrome (PCOS) patients with euploid miscarriage group (n = 7). B and C, Gray scale analysis of Fig. 6A. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control. D, Representative immunofluorescence images showed the morphologic change of cytoskeleton actin filament (green) in HTR-8/SVneo cells with or without knockdown of TFAM. Nucleus were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue). Scale bar: 20 μm. E, Western blot analysis was conducted to measure the expression of N-cadherin and vimentin in HTR-8/SVneo cells with or without knockdown of TFAM. F and G, Gray scale analysis of Fig. 6E. GAPDH was used as the loading control. H, Western blot detection of E-cadherin in BeWo cells treated with or without forskolin. I, Gray scale analysis of Fig. 6H. GAPDH was used as the loading control. J, Western blot detection of E-cadherin in BeWo cells with or without TFAM knockdown and forskolin. K, Quantified protein levels of E-cadherin, relative to the protein levels of GAPDH. *P less than .05; **P less than .01; ***P less than .001.