The effects of TNFα on apoptosis-related gene expression in human mature adipocytes. A, Adipocytes were treated with TNFα (0–10 nmol/L) for 24 h. mRNA expression was normalized against GAPDH and 18S after RT-MPCR and parallel 18S-PCR. P < 0.01 at 3 nmol/L and above for each gene. B, The time course of TNFα effects. P < 0.01 at 24 h.