Challenges and strategies in the application of assisted reproductive technology (ART) in nonhuman primates (NHPs): review

Nonhuman primates (NHPs) have become important models for biomedical research due to their similarities to humans in evolution, anatomy, and physiology. It is worth noting that NHPs have important clinical significance in the field of assisted reproductive technology (ART) research and are an important experimental basis for studying human reproductive physiology and pathogenic mechanisms. However, due to the differences in reproductive physiology between the two species, the application of human ART treatment strategies in NHPs often leads to different experimental results. Therefore, this review provides an analysis of the challenges encountered by NHPs in the application of ART and their possible solutions. This article discusses the technical status and difficulties of semen collection, quality assessment, egg collection, artificial insemination and gamete development in NHP. Based on the analysis of existing technologies, suggestions are put forward to optimize the assisted reproductive technology of NHPs, in order to improve the research model system of early embryo development of NHP. This study contributes to a deeper understanding of NHP and human embryo development, and provides some potential suggestions for optimizing NHP embryo culture and human infertility treatment. The optimization of NHP-ART has important scientific value and application prospects, and in-depth research can make more contributions to the progress of reproductive biology and clinical treatment. This review summarizes and analyzes the problems and strategies that deserve attention in some NHPs-ART technologies, so as to provide reference for researchers in related fields.